Journal: Cancer Immunology, Immunotherapy : CII

Article Title: FTY720 and SEW2871 reverse the inhibitory effect of S1P on natural killer cell mediated lysis of K562 tumor cells and dendritic cells but not on cytokine release

doi: 10.1007/s00262-009-0775-7

Figure Lengend Snippet: Expression of NK cytotoxicity or inhibitory receptors on NK cells before and after incubation with S1P. IL-2-activated NK cells were either left untreated or were incubated with 2 μM S1P for 4 h. These cells were washed and then examined for the expression of activating receptors NKG2D (a), NKp30 (b) or NKp44 (c). Staining with PE-conjugated isotype control (mouse IgG) is also shown. Panels d–f show expression of KIR/CD158 after incubation with 0.02, 0.2 or 2 μM S1P for 4 h, respectively. The expressions of CD158 on S1P-untreated NK cells as well as staining with isotype FITC-conjugated IgG are also shown

Article Snippet: They were labeled with 1 μg/ml FITC-conjugated mouse anti-human CD80, 1 μg/ml FITC-conjugated mouse anti-human CD83, 1 μg/ml FITC-conjugated mouse anti-human CD86 (Becton–Dickinson Pharmingen, San Diego CA, USA), 1 μg/ml FITC-conjugated mouse anti-human HLA-class I, 1 μg/ml FITC-conjugated mouse anti-human HLA-DR, 1 μg/ml FITC-conjugated mouse anti-human HLA-E (ImmunoTools, Friesoythe, Germany), or 1 μg/ml FITC-conjugated mouse IgG (Becton–Dickinson Biosciences-Pharmingen, San Jose, CA, USA) as a control.

Techniques: Expressing, Incubation, Staining

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: FTY720 and SEW2871 reverse the inhibitory effect of S1P on natural killer cell mediated lysis of K562 tumor cells and dendritic cells but not on cytokine release

doi: 10.1007/s00262-009-0775-7

Figure Lengend Snippet: Expression of MHC class I and II molecules and their mean fluorescence intensity (MFI) on iDCs before and after incubation with S1P. Immature DCs were either left untreated or were incubated with 2 μM S1P, 10 nM FTY720, 10 nM SEW2871 or their combinations for 4 h. These cells were washed and then examined for the expression of HLA-DR, HLA-I and HLA-E. Mean ± SEM of percentages of positive cells isolated from three different donors (a–c). Mean ± SEM of mean fluorescence intensity (MFI) of positive cells isolated from three different donors (d–f)

Article Snippet: They were labeled with 1 μg/ml FITC-conjugated mouse anti-human CD80, 1 μg/ml FITC-conjugated mouse anti-human CD83, 1 μg/ml FITC-conjugated mouse anti-human CD86 (Becton–Dickinson Pharmingen, San Diego CA, USA), 1 μg/ml FITC-conjugated mouse anti-human HLA-class I, 1 μg/ml FITC-conjugated mouse anti-human HLA-DR, 1 μg/ml FITC-conjugated mouse anti-human HLA-E (ImmunoTools, Friesoythe, Germany), or 1 μg/ml FITC-conjugated mouse IgG (Becton–Dickinson Biosciences-Pharmingen, San Jose, CA, USA) as a control.

Techniques: Expressing, Fluorescence, Incubation, Isolation

Journal: mAbs

Article Title: Phage display-derived human antibodies in clinical development and therapy

doi: 10.1080/19420862.2016.1212149

Figure Lengend Snippet: Phage display-derived antibodies and antibody conjugates evaluated in clinical studies, including those approved by FDA or EMA.

Article Snippet: BAY1093884 , — , Tissue factor pathway inhibitor (TFPI) , synthetic, Fab , MorphoSys , IgG2 , Bayer , Phase 1 , Hemophilia.

Techniques: Selection, Migration, Virus